Leukaemia Panel, ALL, by FISH Test: Booking, Price, and Results
About Leukaemia Panel, ALL, by FISH Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | ALL FISH Panel, Acute Lymphoblastic Leukaemia Panel by FISH, Leukaemia FISH Panel (ALL), Interphase FISH for ALL, B-ALL FISH Panel |
| Sample Type | Bone marrow aspirate |
| Fasting Required | Not required for local anaesthesia; fasting needed if general anaesthesia is used (common in children) |
| Report Time | 2 to 5 days |
| Recommended For | Children and adults of both genders with suspected or confirmed acute lymphoblastic leukaemia |
| Price | Starting at ₹14,500 |
What is a Leukaemia Panel, ALL, by FISH Test?
The Leukaemia FISH Panel is a specialised genetic test used to detect specific chromosomal abnormalities in leukaemia cells. FISH stands for Interphase Fluorescence in situ Hybridisation, a technique that uses fluorescent probes to identify gene changes. Doctors typically order this test when acute lymphoblastic leukaemia (ALL) is suspected or has been diagnosed, to understand the exact genetic subtype. The sample is usually a bone marrow aspirate, though peripheral blood may also be used.
What Does a Leukaemia Panel, ALL, by FISH Test Measure?
The ALL by FISH panel targets specific gene rearrangements and chromosomal changes that are clinically significant in acute lymphoblastic leukaemia (ALL). The table below lists the parameters included.
| Parameter | What It Detects |
|---|---|
| BCR/ABL1 t(9;22) | The Philadelphia chromosome, a high-risk gene fusion |
| ETV6/RUNX1 t(12;21) | A gene rearrangement is often linked to better outcomes |
| KMT2A (MLL) 11q23 | Rearrangements associated with specific ALL subtypes |
| TCF3 (E2A) | Translocations involving the TCF3 gene |
| Chromosome gains (4, 6, 10, 17) | Extra chromosome copies indicating hyperdiploid ALL |
Why is a Leukaemia Panel, ALL, by FISH Test Done?
This test is ordered when a doctor needs to confirm an ALL diagnosis, classify the subtype, or guide treatment decisions. It provides information that cannot be obtained from a routine blood test alone.
Common Symptoms That May Require This Test
A doctor may order the ALL FISH Panel when a patient presents with the following symptoms:
- Persistent tiredness or weakness due to anaemia (low red blood cell count)
- Unexplained fever or frequent infections
- Easy bleeding and bruising with red spots or purplish discolouration
- Bone pain, particularly in the legs and joints
- Frequent infections
- Heavy menstrual bleeding in women
- Abdominal swelling caused by an enlarged liver or spleen
- Unintended weight loss
- Shortness of breath
- Feeling of dizziness and headaches
Conditions This Test Can Help Detect
The Leukaemia FISH Panel can help identify the following conditions:
- Acute lymphoblastic leukaemia (ALL), a fast-growing cancer of immature white blood cells
- B-cell acute lymphoblastic leukaemia or lymphoma (B-ALL)
- Philadelphia chromosome-positive ALL (BCR-ABL1 fusion)
- ETV6::RUNX1 fusion-positive ALL, associated with favourable outcomes
- KMT2A-rearranged ALL and TCF3::PBX1 fusion-positive ALL
- High hyperdiploid ALL, the most common chromosomal abnormality pattern in childhood B-ALL
Leukaemia Panel, ALL, by FISH Test for Chronic Disease Monitoring
Once ALL has been diagnosed and treated, the Interphase Fluorescence in situ Hybridisation (FISH) panel may be repeated at key treatment milestones. It helps assess how well the treatment is working and can detect minimal residual disease, meaning very small numbers of leukaemia cells that may remain after therapy. If a patient shows signs of relapse, targeted FISH probes based on the original abnormalities can be re-evaluated.
How to Prepare and What to Expect
Preparation for this test depends on the type of anaesthesia used for bone marrow collection. Here is what to know before your appointment.
Do You Need to Fast?
No fasting is required if the bone marrow aspiration is performed under local anaesthesia. However, if the procedure is performed under general anaesthesia, as is common in children, fasting for a few hours beforehand is necessary. Your doctor will give you specific instructions.
Practical Tips Before Your Test
Keep the following points in mind before going for the procedure:
- Tell your doctor about all medicines you take, especially blood thinners such as warfarin, apixaban, or heparin
- Inform the healthcare team of any known allergies
- Wear loose, comfortable clothing that allows easy access to your hip area
- Arrange for a family member or friend to accompany you and take you home afterwards
- Eat and drink normally if local anaesthesia is being used
Step-by-Step Procedure
- You will be asked to lie in a specific position to allow access to the back of your hip bone.
- The area is cleaned with an antiseptic, and a local anaesthetic is injected to numb the site.
- Once the area is numb, a needle is carefully inserted through the skin into the bone.
- A small amount of bone marrow fluid is drawn out using a syringe attached to the needle.
- The needle is removed, and a sterile dressing is applied with gentle pressure to the site. The procedure usually takes only a few minutes.
- The bone marrow sample is labelled and sent to the laboratory for Interphase FISH analysis.
Factors That Can Affect Accuracy
- Poor sample quality or an insufficient number of cells in the specimen
- Improper handling or storage (the sample must not be frozen and should be processed promptly)
- Delays in transporting the sample to the laboratory
- The panel only detects the specific gene changes it is designed to test; other chromosomal abnormalities outside these targets will not be identified
Understanding Your Leukaemia Panel, ALL, by FISH Test Results
Results from the Leukaemia FISH Panel must always be reviewed by a qualified haematologist or oncologist alongside your full clinical picture. The table below provides a general guide to what each result may indicate.
| Parameter | Normal Finding | Abnormal Finding |
|---|---|---|
| BCR-ABL1 t(9;22) | Negative (no fusion signal) | Positive indicates Philadelphia chromosome-positive ALL |
| ETV6-RUNX1 t(12;21) | Negative (no rearrangement) | Positive indicates a favourable-prognosis subtype |
| KMT2A (MLL) 11q23 | Negative (no rearrangement) | Positive indicates MLL-rearranged ALL |
| TCF3 (E2A) | Negative (no rearrangement) | Positive indicates E2A-rearranged ALL |
| Chromosome gains (4, 6, 10, 17) | Two copies of each chromosome | Trisomy or tetrasomy indicates hyperdiploid ALL |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
The ALL by FISH panel targets only specific gene regions covered by its probes. Chromosomal changes outside these targeted regions will not appear in the report. For this reason, conventional chromosome analysis (karyotyping) is often performed alongside FISH testing to provide a broader picture of any genetic abnormalities present.
How to Maintain Healthy Levels
Because this is a diagnostic test for a specific cancer, general lifestyle tips for keeping levels within range do not apply in the usual sense. The following guidance is relevant instead:
- Follow your haematologist-oncologist's recommended schedule for repeat testing and check-ups
- Report any new or returning symptoms, such as fatigue, fever, or bruising, to your healthcare team promptly
- Attend all follow-up appointments to allow timely monitoring of treatment response
Lupin Diagnostics Leukaemia Panel, ALL, by FISH Test Price
This test requires a visit to a Lupin Diagnostics centre. Home collection is not available for the Leukaemia FISH Panel, as the bone marrow aspiration procedure must be carried out in a clinical setting by a trained professional.
| City | Approximate Price (₹) |
|---|---|
| Mumbai | ₹14,500 |
| Bhubaneshwar | ₹14,500 |
| Hyderabad | ₹14,500 |
| Bangalore | ₹14,500 |
| Chennai | ₹14,500 |
| Kolkata | ₹14,500 |
| Pune | ₹14,500 |
| Guwahati | ₹14,500 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The ALL FISH Panel is a genetic test that uses fluorescent probes to detect specific chromosomal and gene rearrangements in leukaemia cells. It helps doctors identify the exact subtype of acute lymphoblastic leukaemia, predict likely outcomes, and decide on the most appropriate treatment approach.
The Leukaemia FISH Panel is ordered when a doctor suspects or has confirmed a diagnosis of ALL. It goes beyond standard blood tests by identifying specific genetic changes that influence how the disease will behave and which treatments are likely to work best.
The preferred sample is bone marrow aspirate, usually 1 to 2 mL collected in a sodium heparin tube. Peripheral blood (2 to 5 mL) is an acceptable alternative in certain situations. Your doctor will advise which sample type is appropriate for you.
The typical turnaround time for the Interphase Fluorescence in situ Hybridisation (FISH) test is 2 to 5 days. Results may occasionally take longer if additional testing is needed to clarify a finding.
A positive BCR-ABL1 result means the Philadelphia chromosome is present. This is seen in roughly 1 to 3% of paediatric B-ALL cases and around 25% of adult B-ALL cases. While it is associated with a higher-risk disease, targeted therapies are available for this subtype. Your doctor will explain what this means for your specific situation.
A positive ETV6-RUNX1 result, caused by the t(12;21) rearrangement, is the most common cytogenetic change seen in paediatric B-ALL. It is generally associated with a favourable outlook. Your haematologist will discuss how this finding fits into your overall treatment plan.
No. The ALL by FISH panel identifies only the specific gene changes it is designed to test. Any chromosomal abnormality outside the targeted probe regions will not be detected. Conventional chromosome analysis and other molecular tests may be recommended alongside this panel to give a more complete picture.
Leukaemia Panel, ALL, by FISH Test: Booking, Price, and Results
